By J. Kroschel (auth.), Jürgen Kroschel (eds.)
Parasitic weeds of the households Cuscutaceae, Orobanchaceae and Scrophulariaceae are thought of to be one of the significant difficulties dealing with agriculture within the Tropics and Subtropics. within the final a long time, huge, immense efforts were made and good fortune accomplished by way of scientists worldwide in gaining a greater realizing in their biology and ecology in addition to of regulate tools. besides the fact that, no gigantic aid of infestation has been completed some time past and regulate innovations particular to different parasites, plants and farming structures needs to be extra constructed or tailored and realised between a much broader farming inhabitants with appropriate extension tools. This `Technical guide' presents updated methodologies for varied features of study and extension concerning parasitic weed species of the genera Striga, Alectra, Orobanche and Cuscuta. It has the goal to aid scientists and extension employees of overseas and nationwide study and extension institutes and universities, who're both new to the topic or plan to use additional suggestions they aren't but ordinary with.
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Extra info for A Technical Manual for Parasitic Weed Research and Extension
Molecular cloning: a laboratory manual, 2nd edn. Vols. 1-3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Williams JGK, AR Kubelic, KJ Livak, JA Rafalski & SV Tingey 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18:6531-6535. 1 Determination of ecological behaviour INTRODUCTION The weed flora in cultivated land is subjected to the diverse and changing aspects of soils and the interrelationships with other plants and other living organisms.
As a prerequisite for the germination of Striga, Alectra and Orobanche seeds, 36 the seeds have to pass a preconditioning period under moist and warm conditions. , from a host root. In the case of parasite seeds a seed is considered germinated when the germ tube has protruded from the seed testa (Fig. 9). - I mm IM G Fig. 9. Development of the germ tube of Orobanche ramosa (on the top) and Striga hermonthica (below). g. g. :fl r th scr ening of trap crops) • to e aluate the p tential of ynthetic germination timulants.
3). Verification of the results can be done by southern hybridisation, that confirm identity of sequences. * COMMENTS This method is very accurate and does not depend on the quality of testa cell walls, that are highly polymorphic in their morphology and rapidly deteriorate when buried in field soil. The RAPD patterns of DNA extracted from soil-borne Orobanche seeds is identical to that of DNA from vegetative plant material provided that the embryo and/or endosperm did not deteriorate. It is therefore recommended to examme 16 soil borne seeds under a dissecting microscope before DNA extraction, and to select for this procedure only seeds that do not look empty.