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Additional resources for Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing: An introduction to the various fields of applications of the in vivo chlorophyll fluorescence also including the proceedings of the fir
Addition of LHC II-peripheral to PSII B yields PSII a . The relative proportion of PSII centers with the three antenna configurations depends on the developmental stage of the chloroplast and on the availability of Chl b. Mature wild type chloroplasts contain PSII a (75-80 0 /0 of the total PSII) and PSII B (20-25 0 /0 of the total PSII). (~) The PSII reducing side heterogeneity refers to the existence of PSII centers with impaired Qj to QB electron transfer interaction (QB-nonreducing centers).
3). 4 ns at F originates from PS II max units which are associated with the predominant part of the Chi alb light-harvesting complex (Holzwarth et al. 1985, Holzwarth 1986a). An example for the corresponding excitation spectra under Fo-conditions is shown for Scenedesmus in Fig. 4. These spectra again reveal that the two PS II fluorescence components with lifetimes of approx. 300 ps and 550 ps show distinctly different excitation spectra. The corresponding PS II units have a different pigment composition in their antenna.
According to these authors, the slower phase of the fl uorescence induction kinetics of chloroplasts in the presence of DCMU is attributed to "l eakage" of electrons from QA (of PSlI s ) to the PQ pool, presumably due to inefficient inhibition by the herbicide. This would result in a slower rate of QA accumulation in these centers. Their hypothesis wa s based on two observati ons: (a) excess ive amounts of DCMU e 1imi na ted the slow exponential phase of the fl uorescence induction curve [Schreiber/Pfister, 1982J and (b) prior addition of dithionite to isolated thylakoids, in sufficient amounts for the chemical reduction of the plastoquinone pool, also eliminated the slow exponential phase of the fluorescence induction [Hodges/Barber, 1983J.